Characterization of Multi-Drug Resistant Gram-Negative Bacteria Present in Fresh Leafy & Salad Vegetables in Dhaka, Bangladesh

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  •   Shahrin Akter Aurin

  •   Sohana Parvin Chowdhury

  •   Maruf Abony

  •   Jarin Rifa

  •   Avijit Banik

  •   Aneeka Nawar Fatema

  •   Oshin Ghurnee

  •   Zakaria Ahmed

Abstract

This study was conducted to determine the microbiological quality of fresh raw and unwashed leafy and salad vegetables in Dhaka, Bangladesh. Six different types of samples (red amaranth, spinach, carrot, radish, tomato, and cucumber) were collected in pre-sterilized zip-lock bags from various local markets in Dhaka. All samples were transported to the Centre of Excellence for Global Health Research Laboratory of Primeasia University at earliest convenience. Samples were enriched in Nutrient Broth media and were then cultured on selective media for isolation purpose. Serial dilution was performed for the total viable count. Biochemical and carbohydrate profiling was conducted for the presumptive identification of the isolates. Commercial antibiotic discs were used for antibiogram by Kirby-Bauer disc diffusion method on Mueller-Hinton agar medium. The total microbial load ranged from 8x107 to 1.70x108. Total 36 isolates were identified, having 7 differentorganisms. The most predominant organism was Vibrio sp. (23%) followed by Klebsiella sp.(20%), Acinetobacter sp. (19%), Pseudomonas sp. (19%), Salmonella sp. (8%), Moraxella sp.(8%) and Escherichia coli (3%). 11% of the Vibrio sp. isolates were V. cholerae, found from 4 samples. No presence of V. cholerae was observed in the tomatosamples. E. coli was observed only in Carrot sample. Antibiotics from 7 different groups were tested against the organisms among which Imipenem showed the highest sensitivity (86%). Following Ceftriaxone (100%), Nitrofurantoin (94%), Erythromycin (89%) and Amoxicillin (83%) had the highest resistance against the isolated organisms. Moreover, most of the isolates showed a multi-drug resistance pattern where they were resistant to at least four drugs. Prevalence of pathogenic bacteria in raw unwashed vegetables can cause potential adverse health effects and therefore the consumers need to be conscious about the matter.


Keywords: Fresh raw vegetables, Food-borne illness, Gram-negative bacteria, Multi-drug resistance

References

Slavin, J.L. and Lloyd B. 2012, Health Benefits of Fruits and Vegetables, Adv Nutr, pp. 506–516.

Dias, J. 2012, Nutritional Quality and Health Benefits of Vegetables: A Review. Food and Nutrition Sciences, pp. 1354-1374.

Zhang, Y.Z., Gan R.Y., Li S., Zhou Y., Li A.N., Xu D.P., and Li H.B., 2015, Antioxidant Phytochemicals for the Prevention and Treatment of Chronic Diseases. Molecules, pp. 21138–21156.

Country Nutrition Paper Bangladesh. Icn, International Conference On Nutrition. s.l.: FAO, 2014.

Younus M.I., Sabuj A.A.M., Haque Z.F., Sayem S. M., Majumder S.,Parvin M.S., Islam M.A., and Saha S. 2020,Microbial risk assessment of ready-to-eat mixed vegetable salads from different restaurants of Bangladesh Agricultural University campus. J Adv Vet Anim Res., pp. 34–41.

UM, FAO. Improving the safety and quality of fresh fruit and vegetables: Maryland: JIFSAN, University of Maryland, Symons Hall, 2002.

Bintsis, T. 2017, Foodborne pathogens. AIMS Microbiol, pp. 529–563.

FAO. Foodborne Illnesses and Outbreaks from Fresh Produce. Washington, DC: FAO, 2019.

Noor, R., Feroz F. 2016, Food safety in Bangladesh: A microbiological perspective. Stamford Journal of Microbiology, pp. 1-6.

Ali, A. N. M. A. 2013, Food safety and public health issues in bangladesh: A regulatory concern. European Food and Feed Law Review, pp. 31-40.

Kabir, A., Das, A., & Kabir, M. S. 2015, Incidence of antibiotic resistant pathogenic bacteria in vegetable items sold by local and super shops in Dhaka city. Stamford Journal of Microbiology, pp. 13-18.

Eni, A. O., Ibukunoluwa O., & Oranusi S. 2010, Microbial quality of fruits and vegetables sold in Sango Ota, Nigeria. African Journal of Food Science, pp. 291-296.

Bohaychuk, V. M., Bradbury, R. W., Dimock, R., Fehr, M., Gensler, G. E., King, R. K., Rieve, R. and Romero Barrios. 2010, A microbiological survey of selected Alberta-Grown fresh produce from farmers’ markets in Alberta, Canada. Journal of Food Protection, pp. 415–420.

Banik, A., Abony M., Datta S., and Towhid S.T. 2018, Microbial Status and Multidrug Resistance Pattern of Pathogenic Bacteria Isolated from Street Food in Dhaka City, Bangladesh., Journal of Advances in Microbiology, pp. 1-13.

Akhi, M.A., Banik A., Ghurnee O., Das N. C., Nondi S. and Abony M. 2019, Prevalence and Antibiogram Profiling of Rotten Fruits from Different Areas of Dhaka City, Bangladesh, European Journal of Medicinal Plants, pp. 1-9.

Banik A., Abony M., Datta S. and Towhid S.T.*. 2019, Microbiological Quality of Ready - to - Eat Food from Dhaka, Bangladesh, Current Research in Nutrition and Food Science, pp. 161-168.

Rabbi, F.A., Rabbi F., Runun T., Zaman K.,Rahman M. & Noor R. 2016, Microbiological Quality Assessment of Foods collected from Different Hospitals within Dhaka City. Stamford Journal of Microbiology, pp. 31-36.

Das N.C., Abony M., Banik A., Akhi M.A., Hossain N. & Ahmed Z., 2020, Optimization of Bacteriocin Producing Probiotic Lactobacillus Spp. Isolated from Broiler Chicken Gut. Forefront Journal of Engineering & Technology, pp. 1-8.

Abony, M., Banik A., Shishir M.A., Akter N.J., Uddin M.E., Datta S. 2018, Physico-chemical Characterization of Indigenous Streptomyces and Influence of pH on Antimicrobial Activity. Microbial Bioactives, pp. 059-067.

Francis G. 2002, Effect of vegetables type and antimicrobial dipping on survival and growth of Listeria and E. coli. Int. J. of Food Sci. and Tech, pp. 711-718.

Mrittunjay, S., V. Kumar. 2017, A study on prevalence of microbial contamination on the surface of raw salad vegetables. Biotech.

Swerdlow, D.L. 1992, Waterborne transmission of epidemic cholera in Trujillo, Peru. Lancet, pp. 28-32.

Kumar, V. 2012, Incidence of Salmonella sp. and Listeria monocytogenes in some salad vegetables, which are eaten raw: a study of Dhanbad City, India. Int J Eng Sci Res, pp. 1437–1442.

Mundhada, R. 2006, Bacteriological Quality of Salad Vegetables Sold in Amravati City (India). Journal of Biological Sciences, pp. 28-30.

Bogomolny, E., S. Swift, F. Vanholsbeeck. 2013, Total viable bacterial count using a real time all-fibre spectroscopic system. The Royal Society of Chemistry.

Amorim, AMB de, Nascimento J dos S. 2017, Acinetobacter: An Underrated Foodborne Pathogen? J Infect Dev Ctries, pp. 111-114.

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How to Cite
[1]
Aurin, S.A., Chowdhury, S.P., Abony, M., Rifa, J., Banik, A., Fatema, A.N., Ghurnee, O. and Ahmed, Z. 2020. Characterization of Multi-Drug Resistant Gram-Negative Bacteria Present in Fresh Leafy & Salad Vegetables in Dhaka, Bangladesh. European Journal of Engineering Research and Science. 5, 11 (Nov. 2020), 1322-1327. DOI:https://doi.org/10.24018/ejers.2020.5.11.2212.